What Is The CD44 Antibody And Its Uses?

The CD44 antibody is a glycoprotein that is used in cell adhesion, migration, and cell-to-cell interactions. It is encoded in humans by Chromosome 11 by the CD44 gene and is referred to as a homing cell adhesion molecule (HCAM), as well as the Pgp-1, Hermes antigen, ECM-III, HUTCH-1, and lymphocyte homing receptor.

Functions

Primarily, this antibody helps with a variety of cellular function, which includes tumor metastasis, hematopoiesis, recirculation, homing, and lymphocyte activation. Likewise, it interacts with ligands, including collagens, MMPs, and osteopontin, as well as hyaluronic acid.

How It’s Used In Clinical Situations

This protein can be used to track T-cell development in a patient’s thymus and can also be an indicative marker for the T-cells. Likewise, variations can be markers for cancer stem cells in the prostate and breast. It may also help to increase survival time for those with epithelial ovarian cancer.

More Information

Primarily, it is for research use only and shouldn’t be used in diagnostics or procedures. Applications include Western Blotting and Immunohistochemistry (IHC). The recommended starting protocol for Western Blotting is to dilute it by a ratio of 1:25 and then incubate at room temperature for one hour, though the dilution ratio is an estimate. The IHC procedure is a little more involve and requires that you prepare the specimen, deparaffinize the slides, dilute the antibody, retrieve the antigens, incubate, and wash slides between all the steps. The IHC positive control is for esophageal carcinoma.

It should not be frozen and should be stored at two to eight degrees Celsius. Because of its natural, you should test both negative and positive controls simultaneously, especially with unknown specimens.

The CD44 antibody is a protein found in the human body and can be used to help research facilities. Visit Spring Bioscience at www.springbio.com now to find out more.

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